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A hexapeptide that is part of a mouse hormone was hydrolyzed…

Posted byAnonymous June 22, 2021November 27, 2023

Questions

A hexаpeptide thаt is pаrt оf a mоuse hоrmone was hydrolyzed to give the following amino acids (in no particular order): Tyr (Y), Cys (C), Glu (E), Ile (I), Lys (K), Met (M).  a) two cycles of Edman degradation of the intact hexapeptide releases the PTH-amino acids below. Remember that the Edman reagent reacts with the N-terminus amino acid and cleaves it off as a cyclic structure (PTH), where only the R group of the amino acid is recognizable. b) cleaving the intact hexapeptide with cyanogen bromide yields Met and a pentapeptide c) treating the intact hexapeptide with trypsin yields a dipeptide, which contains Tyr and Glu, and a tetrapeptide d) when the intact hexapeptide is treated with carboxypeptidase A, a Tyr residue and a pentapeptide are produced.  Write the sequence of the hexapeptide in the blank below. 

A hexаpeptide thаt is pаrt оf a mоuse hоrmone was hydrolyzed to give the following amino acids (in no particular order): Tyr (Y), Cys (C), Glu (E), Ile (I), Lys (K), Met (M).  a) two cycles of Edman degradation of the intact hexapeptide releases the PTH-amino acids below. Remember that the Edman reagent reacts with the N-terminus amino acid and cleaves it off as a cyclic structure (PTH), where only the R group of the amino acid is recognizable. b) cleaving the intact hexapeptide with cyanogen bromide yields Met and a pentapeptide c) treating the intact hexapeptide with trypsin yields a dipeptide, which contains Tyr and Glu, and a tetrapeptide d) when the intact hexapeptide is treated with carboxypeptidase A, a Tyr residue and a pentapeptide are produced.  Write the sequence of the hexapeptide in the blank below. 

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Tags: Accounting, Basic, qmb,

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