Yоu hаve twо аlleles оf а gene, shown as partial coding sequences of the RNA-like strand. Wild-type: 5’…CTTGCAAGCGAATTC…3’ Mutant: 5’…CTTGCTAGCGAATTC…3’ A. What are the possible mRNA(s), reading frame(s) for the mRNA and corresponding amino acid sequence(s) for the wild-type allele? (20p) B. What are possible consequences of the mutant allele? (10p) C. If you use the restriction enzyme EcoRI (which recognizes 5’ G^AATTC 3’) on the wild-type DNA fragment, how many DNA fragments result from this reaction? Write down the sequence(s). (10p) D. To investigate gene expression of the wild-type and mutant alleles, you plan to generate the cDNA from each. How do you obtain this cDNA, and what would the cDNA sequences look like for both alleles? (10p)
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