Please consider the gel electrophoresis image and the DNA la…
Please consider the gel electrophoresis image and the DNA ladder. In the gel, the DNA ladder was loaded in lane 1 (far left), and the PCR product samples were loaded in lanes 2 through 8. All samples were run in the same thermocycler at the same time. In lane 8, approximately what size (in base pairs) is the DNA fragment shown, and why did the PCR produce a product of that size? In lane 7, there is no PCR product visible. What does that tell us about that PCR, and why might that have happened? Offer suggestions, i.e., possible sources of error. The PCR products in lanes 2-5 and 8 are all from different species of bacteria. Why are they all the same size? Explain briefly. Figure 1. Gel electrophoresis results of PCR amplification of 16S rDNA from 7 samples of bacteria. Figure 2. 2-log DNA ladder used in Figure 1.
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