SA3b. (4 pts) Many groups are using a western blot for one of their experiments. List the 4 key steps of a western blot.

Which model of Golgi transport involves a ‘treadmill-like’ motion whereby the Golgi compartments form on the cis face, move through the stack, and then break up on the trans face?

Which type of protein sorting involves SRP?

SA3a. (6 pts) Complete the table below using the dropdown menus.    Pulse Chase Assay GFP Labeling How is protein detected? [answer1]  [answer2]  This technique requires. . . [answer3] [answer4] Can tack/follow cells in real time? [answer5] [answer6]  

In receptor-mediated endocytosis, receptor molecules initially project to the outside of the cell. Where do they end up after they enter the cell?

A transporter that pumps two solutes in the same direction is called a. . .

B. (4 pts) What basic role do G Proteins play in cell signaling?

Which of the following is true about protein domains?

4A. (4 pts) A drawing of an antibody is shown below.  Answer each question by referring to parts of the diagram. Answers may be used once, more than once, or not at all. Which part(s) bind to target proteins? [question1] Which parts are variable? [question2] Which part of secondary antibodies gets labeled? [question3] Which part of the primary antibody does the secondary antibody bind to? [question4]    

What type of signaling involves extremely short-range signals that are carried by vesicles and then dumped into a gap or cleft before being received by a neighboring cell?