Which of the following binds specifically to a sequence of interest, thus facilitating the analysis of that sequence?

DNA of interest  is extracted from a clinical sample.  The absorbance at 260 nm is 0.489 and the absorbance at 280 nm is 0.485.  Is this sample of sufficient quality for use in subsequent analysis?  To receive credit, you must show your calculation and concisely explain your assessment with regards to the extracted DNA’s purity.  

TaqMan, molecular beacons, and scorpion-type primers are all used in which of the following procedures? 

Molecular techniques are useful to diagnose infections caused by organisms that are difficult to culture.

Dye terminator sequencing has been performed using capillary electrophoresis to separate the fragments.  ddATP has been labeled with a green fluor, ddTTp with red, ddCTP with blue and ddGTP with black.  The colors are detected in the following order:  red, green, black, red, green blue, red, black, black, green and blue.  What is the sequence of this fragment?

Which of the following is the output from capillary electrophoresis of a fluorescent product? 

A linear DNA molecule with one restriction enzyme recognition site will yield how many fragments when digested with that restriction enzyme?

A DNA polymorphism is a sequence difference compared to a reference standard that is present in at least 50% of the population.

The Southern blot transfer procedure includes:

Concisely explain how you would prepare a 1% agarose gel to visualize extracted DNA.  Be certain to include the essential components, how much of each you need, and the general process.