Researchers interested in studying possible interactions bet…
Researchers interested in studying possible interactions between lamellipodin (Lpd) and Mena performed the experiment below. Lpd-FP4-2Mito is a fusino protein consisting of Lpd, the FP4 domain which has been shown to bind to Mena, and the 2Mito domain which has been shown to bind to the mitochondria. The Lpd-Pro-Mito fusion protein is similar except that the FP4 domain has been replaced with a bunch of prolines that don’t really do anything other than act as a spacer. Look at the immunofluorescence images and indicate which of the following was the most important result of this experiment.
Read DetailsKrause et al. were interested in determining whether the Lam…
Krause et al. were interested in determining whether the Lamellipodin protein (Lpd) was responsible for bringing Mena to the plasma membrane. In one of their experiments, they created a fusion protein in which red fluorescent protein was fused to the Lpd domain “FP4”, which predicted to bind to Mena, and also fused to the 2Mito domain which is predicted to bind to the mitochondria. They transformed this fusion protein into cells and used fluorescent microscopy to track this fusion protein and Mena. This experiment showed that. . .
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