In prokaryotes, in which part of the cell do replication, transcription, and translation occur?

A researcher has a very small sample of DNA and needs to make millions of copies in order to study a specific gene. Because the original amount of DNA is insufficient for experiments like sequencing or cloning, the researcher decides to use a laboratory technique that can rapidly amplify the DNA. Question:Which technique should the researcher use to amplify the DNA, and what is the basic principle behind it?

Which of the following best describes the direction in which the lagging strand is synthesized?

Which of the following processes are involved in gene expression, the mechanism by which information from a gene is used to produce a functional product such as a protein?

Which of the following statements best explains how an inducible gene regulation system controls gene expression?

Which of the following enzymes is responsible for joining two sugar-phosphate backbones of DNA?

A microbiology researcher is studying Serratia marcescens, a bacterium known for producing a red pigment called prodigiosin. The researcher notices that when the bacteria are grown at 25°C, colonies develop a deep red color, but when grown at 37°C, the pigment production is greatly reduced, and colonies appear pale or colorless. The researcher hypothesizes that temperature affects the expression of genes involved in prodigiosin synthesis. Which explanation best accounts for the difference in pigment production at 25°C versus 37°C? [color1] If a mutation occurred in the regulatory region controlling prodigiosin genes, what would be the expected result at 25°C? [color2] Which experiment would best test whether temperature affects transcription or enzyme activity in prodigiosin synthesis? [color3] Why might S. marcescens benefit from producing pigment at lower temperatures but not at human body temperature (37°C)? [color4] If you wanted to engineer a strain of S. marcescens to produce pigment at 37°C, which approach would be most effective? [color5]

Which statement best explains why DNA replication is considered semiconservative?

The dc-dc bi-directional converter of Figure interfaces a 45/50-V battery with a 120/140-V battery bank. The internal emfs are E1=125 V, and E2​=48 V(dc). Both these battery sources have an internal resistance of 0.05Ω each. In the dc steady state, calculate the power-pole duty-ratio Da, ​ if (a) the power into the low-voltage battery terminals is 500 W, and (b) the power out of the low-voltage battery terminals is 1000 W.

As a new sales representative for Madmel Enterprises, you take a long time customer (ACME) out to dinner and drinks.  Before dinner is over, you have shaken hands on a deal to sell the customer nearly a one and a half million dollars worth of industrial equipment.  Madmel Enterprises is the only supplier of this industrial equipment.  You have orally agreed on the essential terms of the deal such as price, quantity, delivery, etc.  In writing up the formal contract the next morning, you discover that you miscalculated the equipment’s price.  Your error could cost Madmel Enterprises $120,000. You telephoned ACME to explain the situation.  ACME insists we have an enforceable contract. This is likely not an enforceable contract because the statute of frauds requires the contract to be in writing.