The Southern blot transfer procedure includes:

Concisely explain how you would prepare a 1% agarose gel to visualize extracted DNA.  Be certain to include the essential components, how much of each you need, and the general process.   

What is the yield of DNA from a sample with a concentration of 280 ug/mL and a volume of 0.5 mL?

This is a closed book, closed note, closed browser quiz.  You can use a blank white board and a calculator on this quiz.  Any indication of academic dishonesty will be submitted to the GW Office of Academic Integrity.  You have one attempt to complete this quiz.

A plasmid has two sites for the restriction enzyme EcoR1 and one BamHI site.  How many fragments will result when you digest the plasmid with both EcoR1 and BamHI?

Which molecule polymerizes to form a support medium through which nucleic acids move? 

What are the arrows labelled “A” pointing to? Midterm Exam Diagram..png

Calculate the yield of total RNA in ug from a 0.5 mL sample diluted 1:20 with an absorbance at 260 nm = 0.397.

Bisulfite sequencing is useful for ________:

Please look at the plasmid image and calculate what size would it be if you remove the rep(pMB1) piece out of this plasmid.  To get full credit explain how you came up with that plasmid size (if you only have a number, you will not get full credit).   pUC18_19 Plasmid.Qz9.jpg