a. Is the abstract below an example of primary or secondary…
a. Is the abstract below an example of primary or secondary research? b. If primary identify the research design utilized and if secondary, identify the type of article (literature review, systematic review, or meta-analysis) c. Explain WHY you chose the type of article/type of research identified in part b. ________________________________________________________________ Lutein, a carotenoid, exhibits various biological activities such as maintaining the health of the eye, skin, heart, and bone. Recently, we found that lutein has dual roles in suppressing bone resorption and promoting bone formation. Osteoclast differentiation was assessed by coculturing primary osteoblasts and bone marrow cells or culturing an osteoclast precursor cell line. Osteoclast differentiation was significantly inhibited by lutein, astaxanthin, and β-cryptoxanthin. In contrast, only lutein promoted osteoblastic calcified bone nodule formation. To elucidate the molecular role of lutein, we functionally analyzed the NF-κB complex, a molecule involved in bone metabolism, especially in osteoclasts. Docking simulations showed that lutein binds to IKK, thus inhibiting the activation of NF-κB. In a cell culture analysis, the phosphorylation of p65, the active form of NF-κB in osteoblasts, was suppressed by lutein treatment. In vivo, a μCT analysis of the bone microarchitecture showed that lutein improves several bone parameters while maintaining bone mass. Lutein is effective in maintaining bone mass by controlling both bone resorption and formation, which is applied to prevent disuse-induced osteoporosis.
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