A scientist has spent months trying to determine the 3D stru…
A scientist has spent months trying to determine the 3D structure of a large membrane protein.However, despite extensive purification, the protein fails to form crystals, making structural analysis by traditional methods impossible. Which approach is most likely to yield a high-resolution structure without requiring crystals?
Read DetailsYou are purifying a protein that carries a net positive char…
You are purifying a protein that carries a net positive charge at physiological pH (~7.4). The chromatography buffer is maintained at pH 7.4 throughout the experiment. You have access to both cation- and anion-exchange chromatography systems: Cation exchanger: Negatively charged stationary phase (column) Binds positively charged molecules Anion exchanger: Positively charged stationary phase (column) Binds negatively charged molecules If your goal is to retain the protein on the column during purification, which approach should you choose, and why?
Read DetailsScenario: A patient with a spinal cord injury has grade 5 hi…
Scenario: A patient with a spinal cord injury has grade 5 hip flexors, grade 4 quads, and grade 1 dorsiflexion on the right side. The patient has grade 5 hip flexors, grade 3 quads, and grade 3 dorsiflexion on the left side. Patient has no sensation L4 and below on the right side and no sensation L5 and below on the left side. Answer the next 3 questions based on the above scenario What is this patient’s motor level on the right side?
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