Which of the following best describes audience adaptation?

Each recombinant protein expression system has advantages and disadvantages. For each of the following expression systems list two advantages and two disadvantages: E. coli (gram negative bacteria), Saccharomyces cerevisiae (yeast), Trichoderma reesei (filamentous fungus) & mammalian (CHO) cells.  Provide your answer in the following format shown for E. coli as example: E. coli Advantages: text text Disadvantages: text text  

If a student is unsuccessful (drop, withdrawal, failure with grade

Which violations of the Code of Conduct will result in program dismissal (even on first offense)?

What is the minimum passing grade required for this course (based on LSBPNE guidelines)?

Enzymes are produced on a large scale for different applications. For each of the applications below choose an enzyme from the drop down menu.   In the baking industry, the following enzyme[bakingenzyme1], is used to increase dough rising. For high fructose corn syrup production, the following enzyme is the key biocatalyst to make this sweetener[HFSenzyme]. Several enzymes are used in meat processing, one enzyme in particular is now used to create processed meat products (“blocks”) from pieces of meat[meatglue] For “degumming” during juice production this enzyme[fruit] is important for preventing clogging of processing equipment. Finally, this particular enzyme[depilling]  is used in the textile industry to create the stone washed effect of jeans and in laundry detergents for de-pilling of clothes.

You have been asked to express protein X in E. coli. Upon cloning the gene encoding this protein into your favorite expression vector, you realize that you first of all have a hard time cloning (obtaining only few transformants) and that cells transformed with the plasmid (that you finally managed to make), grow very slowly and cultures never reach high cell densities.  Briefly answer the following two questions: 1. What is the most likely reason for your difficulties? 2. How can you overcome this challenge? Name three strategies that you could use to solve your problem.

What makes the industrial use of oxidoreductase enzymes more costly and how is this issue usually dealt with?

Polyketide synthases (PKS) are important biosynthetic assembly lines composed of one or more modules that together synthesize an enormous number of structurally diverse secondary metabolites. A minimal PKS module is composed of 4 essential catalytic domains that catalyze one condensation and subsequent release reaction. Answer questions a-d. a.  Name the 4 domains and catalytic activity in the order that they have to perform their functions. (2 pts) Domain 1: Domain 2: Domain 3: Domain 4: b. Name the typical starter and extender molecules that are linked together by one PKS catalyzed reaction (1 pt)   c. Name the reaction mechanism by which these two molecules are linked together. (1 pt)   d. Write down the structural formula of the final reaction product upon release from the PKS after one reaction round. (1 pt)

Which of the following four text options is correct? Type the corresponding number into the box below. Anaplerotic reaction(s) 1  are not involved in the citric acid production process 2  describe the conversion of glucose to fructose 3  are important for anaerobic fermentation processes 4  have high flux in the glutamic acid production process