You want to insert rfp, a gene coding for red fluorescent protein (RFP), into a plasmid. You cut out a small section of plasmid where you wish to insert rfp by digesting it with the restriction enzymes EcoRI and SacI. What restriction enzyme(s) should you use to digest rfp in order to insert it into your digested plasmid?

After adding the pARA-R (or pGLO) plasmid to a culture of E. coli and then heat shocking them, why did we plate the E. coli out on medium containing ampicillin?

You are using a P-200 that you have set to 0-5-0. You depress the plunger to the second stop, insert your new pipette tip into a solution, and gently release the plunger. Your micropipettor will draw up how much liquid?

In gene expression, which enzyme transcribes the DNA into mRNA?

One of the Mc1r gene mutations you identified in the lab was a silent mutation. What was the contribution of this mutation to the mouse fur color?

You place a drop of blood into a solution and observe it with a microscope. As you watch, the red blood cells expand and begin to lyse. You can correctly conclude the solution you used is _____ relative to the blood cells.

The movement of water from high concentration of water to low concentration of water across a semipermeable membrane is best defined as ______.

A saline IV solution (0.9% salt) for re-hydrating patients is isotonic to human blood. This means human blood is ______ salinity.

Section H-Passato Prossimo with essere-Complete each sentence with the “Passato Prossimo” of the verb in parenthesis.    50-Questi ragazzi _____________________________(nascere) nel 1980.  

72-Tu ________________________(potere) cambiare la prenotazione. (passato)