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Author Archives: Anonymous

How would XmaI (5’-C^CCGGG-3’) be written if added to a reve…

How would XmaI (5’-C^CCGGG-3’) be written if added to a reverse primer?

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Higher percentage agarose gels

Higher percentage agarose gels

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DNA can be detected in an agarose gel by using each of the f…

DNA can be detected in an agarose gel by using each of the following EXCEPT

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Which technique uses PCR to amplify variations in DNA betwee…

Which technique uses PCR to amplify variations in DNA between people (via SNPs, VNTRs, etc.)?

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If you digested the vector below with EcoRI and StuI, how ma…

If you digested the vector below with EcoRI and StuI, how many bands would you see on a gel?

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You can find the colony carrying the gene of interest by usi…

You can find the colony carrying the gene of interest by using all of the following EXCEPT

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What technique is used to increase plasmid copy number and p…

What technique is used to increase plasmid copy number and purify plasmids?

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If a laboratory strain of E.coli cannot grow in the presence…

If a laboratory strain of E.coli cannot grow in the presence of a bacteriostatic compound, it is considered a

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You are working with a vector that has both an ampicillin an…

You are working with a vector that has both an ampicillin and kanamycin resistance gene. If you insert your gene into the multiple cloning site, the colonies will grow on a media that contains

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Which technique is used to amplify a gene when only the part…

Which technique is used to amplify a gene when only the part of the sequence is known, and was important for genome walking?

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