You perform PCR with two primers with melting temperatures of 50 °C and 60 °C. To ensure proper binding, you use an annealing temperature of 30 °C. Which is TRUE?

qPCR quantifies DNA during the

Which of the following is TRUE regarding expression vectors?

Which of the following PCR techniques involves using four or more primers to amplify more than one target in a sample?

If a laboratory E.coli strain requires methionine to survive, it is considered a

qPCR is advantageous over traditional PCR and agarose gel electrophoresis in that

You digest a vector with only EcoRI (5’ overhang), and both ends of a PCR product with EcoRI. Upon ligation of the product into the vector,

T4 DNA polymerase and dCTP are mixed with the dsDNA below. Which of the following is TRUE? 5’-ACCTGTAGCTG… 3’-TGGACATCGAC…

Organic contaminants that remain in a DNA purification can be quantified by measuring the absorbance at

Which is TRUE regarding restriction enzymes?