Use the two diagrams shown above for tetracycline-based gene…
Use the two diagrams shown above for tetracycline-based gene regulatory approaches as a guide for answering the following questions. Note that only transcriptional regulation in the absence of tetracycline is depicted. For each statement below, indicate whether it applies to transgene regulation using the tetR-VP16, RtetR-VP16, both tetR-VP16 and RtetR-VP16, or neither tetR-VP16 or RtetR-VP16 systems. The tetO sequence is located between the promoter that drives transgene expression and the transgene coding sequence [fusion1] The addition of tetracycline represses transcriptional expression of the transgene [fusion2] The VP16 portion of the hybrid transcriptional regulatory protein functions as a transcriptional activator [fusion3] The addition of tetracycline activates transcription of the transgene [fusion4] When the hybrid transcriptional regulatory protein is bound to its operator sequence, transcription occurs [fusion5]
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