20.  Name 2 enzymes responsible for the breakdown of Starch to D-glucose.

How does a high calorie, high protein diet help improve complications of the liver?

23.  These are Lysine decarboxylase media.  Describe the reactions in Tubes A and C only.  After inoculation, we added a reagent to the inoculated tubes , what was that reagent and what was the purpose?

3. You performed an Elisa test in the lab to detect antibody against HIV in the patient’s serum.  The test was found to be 96% sensitive, what does that mean? 

12.  You were able to identify it as Streptococcus.  What is the next thing you would do to speciate it (i.e. into species)?

21.  You are showing a high school student who is shadowing you (following you around) in your lab class, a Light Compound Microscope. Name 3 things you will tell him/her that will help the student to recognize the oil immersion objective lens.

Compare and Contrast (give 1 similarity and 1 difference) between DNA polymerase and Reverse Transcriptase.

Compare the Normal mRNA to the mRNA with mutation sequence below.  Pick the choice that describes the mutation at the DNA level and the protein level.  5′ UUU  CAU  AAG   UAC  AGC 3′  — Normal mRNA 5′ UUU  CAU  AAG   UAA  AGC 3′  — mRNA with mutation  

8.  What is the difference between Phenol red and Neutral red indicators?

18.  Outline the 7 major steps in gram staining (HINT: washing and blotting are not major steps for the purposes of this question), include the time and reason for each step .