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Author Archives: Anonymous

What is the “X-dropoff” parameter in BLAST extension? A) Max…

What is the “X-dropoff” parameter in BLAST extension? A) Maximum number of gaps allowed B) Database size limit C) The score threshold below which extension stops  D) Number of mismatches tolerated

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Why does COBALT perform better than ClustalW for aligning di…

Why does COBALT perform better than ClustalW for aligning distant homologs? A) It’s faster B) It uses domain information as constraints to guide alignment C) It only works with proteins D) It requires fewer sequences

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What does GEO (Gene Expression Omnibus) store? A) Protein st…

What does GEO (Gene Expression Omnibus) store? A) Protein structures B) DNA sequences C) Gene expression data from microarray and RNA-seq experiments D) Clinical variants

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Which NCBI database is considered the “original DNA memory o…

Which NCBI database is considered the “original DNA memory of life”? A) RefSeq B) GenBank C) SRA D) Genome

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Why is the BLOSUM62 matrix called “62”? A) It contains 62 am…

Why is the BLOSUM62 matrix called “62”? A) It contains 62 amino acids B) Sequences with ≥62% identity were clustered during matrix construction C) It was created in 1962 D) It uses 62 scoring parameters

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What is the clinical significance of the EGFR T790M mutation…

What is the clinical significance of the EGFR T790M mutation? A) It makes cancer more aggressive B) It has no clinical relevance C) It is a benign polymorphism D) It confers resistance to first-generation EGFR inhibitors through steric blocking  

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What is the typical length range for good PCR primers? A) 10…

What is the typical length range for good PCR primers? A) 10-15 nucleotides B) 18-24 nucleotides C) 30-40 nucleotides D) 50+ nucleotides

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Why is SRA (Sequence Read Archive) important for scientific…

Why is SRA (Sequence Read Archive) important for scientific reproducibility? A) It stores final analysis results B) It validates all experiments C) It preserves raw sequencing data, allowing independent reanalysis with new algorithms D) It only stores successful experiments

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In the affine gap penalty model, why is gap extension penalt…

In the affine gap penalty model, why is gap extension penalty lower than gap opening penalty? A) Evolutionary evidence shows extending an existing gap is less costly than creating a new one B) It’s computationally easier C) It prevents all gaps D) It speeds up alignment

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What is the primary advantage of using tBLASTn over BLASTp?…

What is the primary advantage of using tBLASTn over BLASTp? A) Finding protein-coding genes in unannotated DNA sequences B) Faster computation C) Higher sensitivity for closely related proteins D) Better for DNA-DNA comparison

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