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Author Archives: Anonymous

A protein has an absorbance of 0.65, and 3 tryptophans (ε =…

A protein has an absorbance of 0.65, and 3 tryptophans (ε = 5500) and 4 tyrosines (ε = 1490). What is its concentration?

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Which of the following is NOT an advantage of the Bradford A…

Which of the following is NOT an advantage of the Bradford Assay?

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How do you prepare 550 ml of 80 mM Tris (Mw = 121.14)?

How do you prepare 550 ml of 80 mM Tris (Mw = 121.14)?

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What sequence of steps should be performed in order to purif…

What sequence of steps should be performed in order to purify and analyze a protein?

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How do you prepare 1 L of 40 mM Tris pH 8 (Mw = 121.14), 600…

How do you prepare 1 L of 40 mM Tris pH 8 (Mw = 121.14), 600 mM NaCl (Mw = 58.44) from a 1 M stock of Tris pH 8? 

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Which of the following is equal to 150 mM?

Which of the following is equal to 150 mM?

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A protein has an absorbance of 0.82, and 2 tryptophans (ε =…

A protein has an absorbance of 0.82, and 2 tryptophans (ε = 5500) and 5 tyrosines (ε = 1490). What is its concentration?

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Which organization requires laboratories to provide a Safety…

Which organization requires laboratories to provide a Safety Data Sheet for all chemicals?

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You are making a 1 M Tris pH 8.0 buffer, and you dissolve th…

You are making a 1 M Tris pH 8.0 buffer, and you dissolve the correct amount of Tris into water and pH it with HCl. If you pH it to 7.9 you should

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The Principal Investigator of the laboratory is usually the…

The Principal Investigator of the laboratory is usually the ________ on a paper.

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