You want to study the binding interaction between a protein…
You want to study the binding interaction between a protein (P) and a ligand (L). The binding equilibrium is: P+L⇌PLP + L \rightleftharpoons PLThe dissociation constant is defined as: Kd=[P][L][PL]K_d = \frac{[P][L]}{[PL]} You perform an experiment where the total concentration of protein [P]T[P]_T is fixed at 10 nM, and the concentration of ligand [L][L] is varied. The fraction of protein bound at each ligand concentration is measured as follows: [L] (nM) Fraction Bound (θ) 2 0.17 5 0.33 10 0.50 20 0.67 50 0.83 Using the data, estimate the dissociation constant (Kd) for the protein–ligand interaction (3 points) with its proper unit (1 point).
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