The mother of a toddler admitted to the hospital for fever o…
The mother of a toddler admitted to the hospital for fever of unknown origin, tells the nurse that she can only stay and visit while her other children are in school. The nurse shows an understanding of a toddler’s psychosocial development by making which statement to the mother?
Read DetailsA scientist has spent months trying to determine the 3D stru…
A scientist has spent months trying to determine the 3D structure of a large membrane protein.However, despite extensive purification, the protein fails to form crystals, making structural analysis by traditional methods impossible. Which approach is most likely to yield a high-resolution structure without requiring crystals?
Read DetailsYou are purifying a protein that carries a net positive char…
You are purifying a protein that carries a net positive charge at physiological pH (~7.4). The chromatography buffer is maintained at pH 7.4 throughout the experiment. You have access to both cation- and anion-exchange chromatography systems: Cation exchanger: Negatively charged stationary phase (column) Binds positively charged molecules Anion exchanger: Positively charged stationary phase (column) Binds negatively charged molecules If your goal is to retain the protein on the column during purification, which approach should you choose, and why?
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