Set 11 – Lab 7: Enzymes Part 1: What substances did you use to test for the optimum pH activity? (3 pts.) Part 2: What other substances around the house do you think you could use as an alternate acid and a base to test for optimum pH? (2 pts.)
Read DetailsTo acidify the lumen of lysosomes (compartments in the cell involved in breakdown and recycling of biomolecules), V-type ATPase transporters are used to pump protons (H+) into the lumen (interior) of the lysosome. If the pH of the inside of the cell (cytosol) is 7.4 (4 x 10-8 M H+) and the luminal pH of the lysosome is 4.0 (1 x 10-4 M H+), calculate the free energy for a mole of protons (H+) to be pumped from the cytosol into the lumen of the lysosome. Note: We can consider, when determining membrane potential, that the movement of a solute molecule from the cytosol into the lumen of a lysosome is the same as movement from the inside of the cell to outside the cell. Assume: ΔGt = RT ln(C2/C1) or ΔGt = RT ln(C2/C1) + Z FΔψ T= 310 K R= 8.315 J/mol*K F= 96,480 J/mol*V (Faraday’s constant) Δψ = −50 mV (membrane potential, inside)
Read DetailsAt the beginning of the year, Activewear Store Inc. had $300,000 of merchandise inventory. During the year, Activewear made inventory purchases of $850,000 and earned a gross profit of $540,000 on sales revenues of $1,200,000. Activewear’s cost of goods sold and ending inventory, respectively, are:
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