Identify the part of the microscope.   

Name two types of personal protective equipment (PPE) you would be required to wear working in a BSL-2 microbiology lab on campus.

What is the advantage of a serial dilution over a direct dilution?

Serial ten-fold dilutions are made from a broth culture of Bacillus subtilis in order to determine the number of bacteria in the culture.  Following the dilution series, 0.1 ml of a 1:100,000 (1 x 10-5) dilution is plated, the plate is incubated, and 87 colonies are counted.  Calculate the number of bacteria in the original Bacillus subtilis culture. Don’t forget units! Cannot earn full credit without proper units. Make sure your answer reflects proper scientific notation! Use the ^ to denote an exponent, i.e. 1 x 10-5 would be written as 1 x 10^-5.

What is the advantage of a serial dilution over a direct dilution?

When preparing a bacterial smear for staining, should you prep a thin or a thick smear? Explain.

When preparing a bacterial smear for staining, should you prep a thin or a thick smear? Explain.

Serial ten-fold dilutions are made from a broth culture of Bacillus subtilis in order to determine the number of bacteria in the culture.  Following the dilution series, 0.1 ml of a 1:100,000 (1 x 10-5) dilution is plated, the plate is incubated, and 87 colonies are counted.  Calculate the number of bacteria in the original Bacillus subtilis culture. Don’t forget units! Cannot earn full credit without proper units. Make sure your answer reflects proper scientific notation! Use the ^ to denote an exponent, i.e. 1 x 10-5 would be written as 1 x 10^-5.

Observe the growth pattern in each tube below:    What is the oxygen class of the organism growing in Tube C? [a] Give an example of an organism (genus and species) that belongs in this oxygen class: [b] What is the oxygen class of the organism growing in Tube E? [c] Give an example of an organism (genus and species) that belongs in this oxygen class: [d]

What is the pH indicator used in MSA plates?