A third promoter is placed to the right of a gene and direct…
A third promoter is placed to the right of a gene and directs RNA polymerase to move leftward. The top DNA strand runs 5′ to 3′ from left to right, and the bottom strand runs 3′ to 5′ from left to right. Complete the transcription analysis.The template strand is the ______ strand. [blank1]The coding strand is the ______ strand. [blank2]The mRNA sequence matches the coding strand except that RNA contains ______ in place of thymine. [blank3]
Read DetailsAssume the gene encodes a 300-amino-acid enzyme, no alternat…
Assume the gene encodes a 300-amino-acid enzyme, no alternative start codon is used, and the catalytic residue is essential. Classify the predicted functional disruption using all information in each row.A synonymous change occurs in the final third of the coding region and does not alter splicing. [row1]A one-nucleotide deletion occurs at codon 2. [row2]Three nucleotides are inserted into a flexible, nonconserved tail, and activity falls to 60 percent. [row3]A missense mutation changes the catalytic residue and leaves less than 5 percent activity. [row4]A conservative surface substitution leaves 85 percent activity. [row5]A nonsense mutation at codon 240 removes a regulatory tail but preserves the catalytic domain. [row6]
Read DetailsResearchers pulse-label newly synthesized DNA in a ligase-de…
Researchers pulse-label newly synthesized DNA in a ligase-deficient strain and separate products on a denaturing gel. Select every pattern that supports a defect in joining Okazaki fragments rather than a defect in origin recognition.
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