A sputum sample submitted for Mycobacterium culture grew numerous orange-colored colonies that stained acid-fast after three weeks of incubation in 37° C in the dark.  These characteristics are consistent with identification of this isolate as a:

Match the organism with the gram-stain reaction and morphology

 In the Kirby Bauer disk diffusion test, if the density of the inoculum used to seed a plate for an antibiotic susceptibility test is less than the standard, the zone diameter will show which of the following after proper incubation?

According to the picture shown here or a special stain from a sputum specimen,  what would be an appropriate genus of an organism that would have this type of staining characteristic? final stain pic.png

A gram-negative rod was isolated from the blood of a patient who spent time in Southeast Asia during the Vietnam war. His clinical condition started with a pulmonary infection that progressed to bacteremia and subsequent septicemia. The organism produced the following morphology on SBA and test results: P. pseudomallei 2.png TSI: alkaline/alkaline Motility: positive Oxidase: positive Oxidative/Fermentation Glucose: oxidative utilization only The MOST probable genus of this isolate is:

Culture GAS.jpg In this photograph are colonies growing on a blood agar plate.  The organism was recovered from throat of a 4 year old child who complained of sore throat and fever. The most likely identification is:

The colonies shown in the image below grew from the CSF taken from a 75 year old male patient who experienced nausea/vomiting, fever, severe headache and stiffness of his neck. Direct smear of the CSF is also shown below. The patient was diagnosed with bacterial meningitis. Based on the information given, which of the following would you suspect as the causative agent? Alpha mucoid(1).jpgCLSC CSF 3(1).jpg

If an Enterobacteriaceae member utilizes sucrose but not lactose, what will the triple sugar iron (TSI) reaction look like?

During a recent hurricane disaster in Puerto Rico, a jaundice 7 year old boy with a history of drinking water taken from a rat-infested pond presents with a flu-like syndrome. Because of the clinical presentation and history, a urine sample was submitted for direct dark-field examination. Several spiral-shaped organisms are seen moving on the slide. The organism also grew in Fletcher’s media.  Which of the following should the physician suspect as responsible for the patient’s condition?

A 35 year old pregnant femalewith fever, chills nausea and vomiting is admitted to the hospital. On further examination, the patient seemed incoherent and confused. CSF and blood cultures are taken and sent to the laboratory for examination.  Gram stained findings on the CSF showed gram positive non-spore forming rods. The colonies produced a narrow zone of beta hemolysis on sheep blood agar; catalase was produced. Based on these findings, you would suspect: