Streak for Isolation: You have been provided with two TSA plates: one new and unused and the other pre-incubated, full of bacterial colonies. Your pre-incubated plate should be labeled either “A” or “B.” You are expected to streak an isolated colony from the pre-incubated plate onto your new TSA plate in the form of a T-streak. Please ensure that you are streaking in the correct direction, changing sterile loops between streaks, and that you have labeled your new TSA plate CORRECTLY. You will be graded on your ability to correctly label the plate (red marker, along the rim, and including all of the key information), as well as your ability to properly streak in the assigned fashion (T-streak). You will get full credit if isolated colonies are present on your plate after 24 hours of incubation, which you can ensure happens by employing proper streaking technique. Please remember that the 3 sections of your T-streak should be labeled correctly (1, 2, or 3) and that you should streak from 1 to 3.  If you turn in a plate with pierced or punctured agar, you will NOT receive full credit for this portion. If you accidentally pierce or puncture your agar, please raise your hand so that we can provide you with a new TSA plate with no penalty. Once you submit your plate, however, you will not have the opportunity to redo this part of the midterm. 

Enumeration of Microbes: Here, you will be expected to calculate the CFU per milliliter in an unknown undiluted sample after being given an image of a Petrifilm, with its dilution factor indicated. Please ensure that your final answer provides the correct UNITS and calculation. 

Kohlering: You are not expected to submit anything here. This will just serve as a placeholder and will be graded based on your in-person performance in this section of the exam.  You will be graded on the maginification of your microscope (should be set to 4x or 10x), the clarity and sharpness of your object (the + sign or circle on your calibration slide, should be centered and focused) and the visibility of your polygon (should be fully in view and edges should be visible and relatively clear/sharp). 

In-person Gram Staining (microscope analysis):  You are not expected to submit anything here. This will just serve as a placeholder and will be graded based on your in-person performance in this section of the exam.  Is your image clear and in focus at 100x (with immersion oil)? Can we clearly see the shape, arrangement, and Gram reaction of the individual cells in your sample?  Please ensure that we can clearly see the cells (sharp and in focus at 100x with immersion oil) and that their shape and arrangement is clear and visible. You are allowed to start over if you are unsatisfied with your results, but please be conscious of time and make sure that your partner is able to use the microscope as well. When you are satisfied with your image, raise your hand and a member of the teaching team will come over and look at your results. They will then score you based on the image in your microscope. 

How does caffeine work? For what type of athletes might it be beneficial and why?       

A college athlete you’re working with is interested in starting to take some supplements. What would be some questions you could ask to help guide this decision-making process based upon the article decision making tree covered in the voiceover lecture? 

In the household measurement system, 8 oz is equivalent to __________.

The metric equivalent of 0.001 gram is __________.

The abbreviation for tablespoon is __________.

The abbreviation for drops is __________.