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The Lineweaver-Burk plot (1/V vs 1/[S]) (Figure 4) below is…

The Lineweaver-Burk plot (1/V vs 1/[S]) (Figure 4) below is a common way to plot Michaelis-Menten data in a linear form in order to determine the kinetic constants Vmax and KM. Figure 4:   a) (4 pts) Calculate the values of Vmax (µM/min) and KM (µM) for a specific enzyme if the equation for the Lineweaver- Burk plot fit to the data is y = 0.224x + 0.0222. Enter the answer on the exam, show all your calculations for full credit on the worksheet. Use units where appropriate. b) (4 pts) Calculate the values of Vmax (µM/min) and KM (µM) for the same enzyme in the presence of an inhibitor, if the equation for the Lineweaver-Burk plot fit to the data is y = 0.6663x + 0.0222. Enter the answer on the exam, show all your calculations for full credit on the worksheet. Use units where appropriate. c) (2 pts) Based on the data, what type of inhibition is this? Explain your answer. d) (7 pts) Calculate kcat (in s-1) for this enzyme in the absence of inhibitor, if the above data is obtained in the presence of 75 nM enzyme and the enzyme has 2 active sites. Be careful with unit conversions! Enter the answer on the exam, show all your calculations for full credit on the worksheet. Use units where appropriate.

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