53-59. Multiple Choice Please choose the BEST answer

The nurse is assessing a patient who was brought by a friend to the ED after a significant mountain bike crash. The patient is minimally conscious and the nurse notices paradoxical breathing with bruising over the right side of the chest. The nurses suspects what injury? 

An electrical worker sustained significant traumatic injuries after falling from a height when his saftey equipment failed. The paramedics are on scene and are deciding what facility would best meet the patient’s needs. What facility would be the best choice for the circumstances? 

Which are the 3 highest priorities while conducting a primary patient survey during the emergency assessment? Select all that apply.

The nurse understands that when a mass casualty incident occurs, that the patients often arrive in “waves.” This is understood to be the case because of which of the following?

The nurse is preparing education for the Emergency Department staff on a suspected new endemic virus that the WHO predicts will become a pandemic. What is the priority teaching in regards to staff safety? 

The nurse is assessing the deformity to the patient’s left thigh. What is the priority concern? 

A new graduate nurse working in the Emergency Department is nervous about triage training in the future. Which statement below from a charge nurse is true about the process of ED triage? 

A patient is admitted to the Emergency Department with thermal burns after a structure fire. The TBSA is 42%, patient weight is 102 kg. Per the fluid resuscitation guidelines of 2-4 mL/kg x TBSA, what is the minimum amount of IV fluid that the patient should receive in the next 24 hours?  (Numeric answer only, no “mL.” Round to nearest whole number if applicable)

Answer the following questions about CRISPR/Cas9 using the information provided in class: a) What is the natural function of CRISRP/Cas and where is it found? (1 pt) b) Annotate the following diagram showing the engineered CRISPR/Cas9 system by filling in the missing information for the numbers in the table below (4 pts)    Answer 1: 2: 3: 4: 5: 6: 7: 8:   c) Explain how the engineered CRISPR/Cas9 system differs from the natural system. Specifically, how the natural system was adapted for engineering purposes and what had to be done to get this bacterial system to function in eukaryotic cells? (3 pts) d) CRISPR/Cas9 typically generates short insertions and deletions at a target site via which repair target pathway in eukaryotic cells? (1 pt) e) Which mechanism would you need to exploit to achieve site-specific DNA insertion into a CRISRPR/Cas9 target site? Explain how this would work for replacing e.g. a gene or introducing a mutation. Can you think of a strategy to increase the efficiency that this will happen? (2 pts) f) What needs to be present in a bacterial cell for efficient DNA/gene insertion using CRISPR/Cas9? (1 pt) g) What does dCas9 stand for and give three different examples on how dCas9 is used? (3 pts)