PCRs will be set up to identify possible R.equi infections i…
PCRs will be set up to identify possible R.equi infections in 20 blood samples via the detection of a choeE gene sequence that is unique to the organism. A master mix is to be set up for 21 samples, with the volumes of most components below. a) Calculate the volume of 10X buffer and water needed per sample, and therefore how much is required for the x21 master mix. (3 marks) b) The products are to be visualised on an agarose gel. Calculate how much of each of the following four components is required to prepare 200 ml of a 1.5% weight/volume agarose gel. Clearly state the units in your answers. 10X TAE buffer Agarose powder 10,000X Gel Red solution Water (2 marks) c) The total volume of each PCR is 50 m What volume of 6X loading buffer needs to be added to each sample before they can be run on the gel? (1 mark)
Read DetailsInstructions: Please compose a literary analysis essay on Ch…
Instructions: Please compose a literary analysis essay on Charlotte Perkins Stetson’s short story “The Yellow Wallpaper.” Your essay should be based on the outline that you completed. Please type your essay in the Canvas textbox. Because the textbox has limited functions, do not worry about double spacing the essay. Documentation: In-text Citation: Please use the page numbers that are listed at the top of the story for your page numbers in the parenthetical citations. For the exam essay, your parenthetical citations should consist of the author’s last name and page number: (Stetson #). Work Cited: Drop down to a new line beneath the conclusion paragraph to begin the Work Cited page. Type the citation below on the Work Cited page. Stetson, Charlotte Perkins. “The Yellow Wallpaper.” 1892. PDF. Length requirement: 5 paragraphs (introduction, 3 body paragraphs, and conclusion) Escue Outline.pdf The-Yellow-Wall-Paper.pdf
Read DetailsInstructions: Please compose a literary analysis essay on Ch…
Instructions: Please compose a literary analysis essay on Charlotte Perkins Stetson’s short story “The Yellow Wallpaper.” Your essay should be based on the outline that you completed. Please type your essay in the Canvas textbox. Because the textbox has limited functions, do not worry about double spacing the essay. Documentation: In-text Citation: Please use the page numbers that are listed at the top of the story for your page numbers in the parenthetical citations. For the exam essay, your parenthetical citations should consist of the author’s last name and page number: (Stetson #). Work Cited: Drop down to a new line beneath the conclusion paragraph to begin the Work Cited page. Type the citation below on the Work Cited page. Stetson, Charlotte Perkins. “The Yellow Wallpaper.” 1892. PDF. Length requirement: 5 paragraphs (introduction, 3 body paragraphs, and conclusion) Flores Outline.pdf The-Yellow-Wall-Paper.pdf
Read DetailsInstructions: Please compose a literary analysis essay on Ch…
Instructions: Please compose a literary analysis essay on Charlotte Perkins Stetson’s short story “The Yellow Wallpaper.” Your essay should be based on the outline that you completed. Please type your essay in the Canvas textbox. Because the textbox has limited functions, do not worry about double spacing the essay. Documentation: In-text Citation: Please use the page numbers that are listed at the top of the story for your page numbers in the parenthetical citations. For the exam essay, your parenthetical citations should consist of the author’s last name and page number: (Stetson #). 2. Work Cited: Drop down to a new line beneath the conclusion paragraph to begin the Work Cited page. Type the citation below on the Work Cited page. Stetson, Charlotte Perkins. “The Yellow Wallpaper.” 1892. PDF. Length requirement: 5 paragraphs (introduction, 3 body paragraphs, and conclusion) Campbell Outline.pdf The-Yellow-Wall-Paper.pdf
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