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Please consider the gel electrophoresis image and the DNA ladder. In the gel, the DNA ladder was loaded in lane 1 (far left), and the PCR product samples were loaded in lanes 2 through 8. All samples were run in the same thermocycler at the same time. In lane 8, approximately what size (in base pairs) is the DNA fragment shown, and why did the PCR produce a product of that size? In lane 7, there is no PCR product visible. What does that tell us about that PCR, and why might that have happened? Offer suggestions, i.e., possible sources of error. The PCR products in lanes 2-5 and 8 are all from different species of bacteria. Why are they all the same size? Explain briefly. Figure 1. Gel electrophoresis results of PCR amplification of 16S rDNA from 7 samples of bacteria. Figure 2. 2-log DNA ladder used in Figure 1.

A pregnant woman has been receiving a magnesium sulfate infusion for treatment of severe preeclampsia for 24 hours. On assessment the nurse finds the following vital signs: temperature of 37.3° C (99.1°F), pulse rate of 88 beats/min, respiratory rate of 10 breaths/min, blood pressure (BP) of 148/90 mm Hg, absent deep tendon reflexes, and no ankle clonus. The patient complains, “I’m so thirsty and warm.” The nurse

Magnesium sulfate is given to women with preeclampsia and eclampsia to

The clinic nurse is performing a prenatal assessment on a pregnant client at risk for preeclampsia.  Which clinical sign is not included as a symptom of preeclampsia?

The nurse is reviewing a list of foods high in folic acid with a patient who is considering becoming pregnant. The nurse determines that the patient understands the teaching when the patient states she will include which list of foods in her diet?

For the client who delivered at 9:02AM on January 14, Rho(D) immune globulin (RhoGAM) must be administered prior to:

A pregnant client’s mother is worried that her daughter is not “big enough” at 20 weeks of gestation. The nurse palpates and measures the fundal height at 20 cm, which is even with the woman’s umbilicus. Which should the nurse report to the client and her mother?

A female client presents to the emergency room complaining of lower abdominal cramping with scant bleeding of approximately 2 days duration. This morning, the quality and location of the pain changed, and she is now experiencing pain in her shoulder. The client’s last menstrual period was 28 days ago, but she reports that her cycle is variable, ranging from 21 to 45 days.  Which clinical diagnosis does the nurse suspect?

You set up three separate test tubes containing equal amounts of glucose along with appropriate media setup and conditions to support growth as follows: aerobic respiration, anaerobic respiration, and fermentation. You inoculate each with E. coli, which is capable of growth using any of these approaches under the appropriate conditions. You proceed to measure the optical density of each culture using a spectrophotometer by way of measuring their growth. Which culture should reach the highest optical density, i.e., the greatest turbidity, representing the most cells produced? Copyright 2025 by Dr. Jonathan A. Miller. All rights reserved. Online sharing or distribution is prohibited. For exam use only in BIOL& 260: Microbiology at Edmonds College. Outside help is not allowed.