In order to characterize the entire promoter for gene X, res…
In order to characterize the entire promoter for gene X, researchers cloned three regions of DNA that based upon their position and sequence could serve as a core promoter, a proximal promoter, or an enhancer element. They subcloned each these regions into luciferase reporter constructs as described and depicted below. Construct A represents the luciferase vector without any promoter elements. Construct B represents the element in its normal position and orientation. Construct C represents the element in its normal position, but in the reverse orientation. Construct D represents the element in a different position, but its normal orientation. Construct E represents the element in a different position AND in the reverse orientation. The results below are for one of the three elements tested. The darker color represents samples that had luciferase activity. Assuming all the control and additional requirements for transcription are correct and met, the test below represents [element] because it is position [reason1] and orientation [reason2]. .
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