You are looking at results from a flow cytometry experiment with two plots, A and B., representing the same results. At first glance A             B

For affinity purification of a protein from a bacterial lysate, which of the following collections would you expect to see the highest concentration of your desired (tagged) protein?

You recorded a flow cytometry experiment with a sample that had several cell populations in it. Plotting FSC vs. SSC which population (labeled in the graph as 1, 2 or 3) in this graph are the largest?  

What is the function of the blocking step in Western blotting?

When designing an expression plasmid for the gene assigned to your group, it is essential that:

Which region of an antibody is responsible for antigen binding?

Why might a primary antibody to your protein of interest interfere with co-immunoprecipitation experiments?

Download the .docx and .xlsx files and follow given instructions.  6304 Module 7 Assignment.docx 6304 Module 7 Assignment Data.xlsx

What is the first step in a typical three-phase protein purification strategy?

Which of these are necessary when giving an indirect quote? (choose all that apply)