You are investigating the effect of a novel protein, HERKY3, on the interaction kinetics of the interaction between two other proteins, HAWKS and RULE. First, you run an experiment where HAWKS is immobilized on a chip and RULE is flowed in (solid line). Then, you repeat the experiment, but in the presence of HERKY3. The addition of HERKY3 has one effect: it causes the dissociation rate of HAWKS and RULE to decrease. Which sensorgram (dashed line) corresponds to this result when compared to HAWKS-RULE alone (solid line)? Note that all dashed lines are slightly raised from the solid lines to differentiate them visually.

IMAGING

Give two approaches that can be implemented to solve the mass transport limitation

Fill in the blanks: Phase contrast microscopy converts a difference in [variable1] to a difference in [variable2].

OK, what question would you have included on the exam that did not appear? Write your question and the answer you would have provided.

In the treatment of cardiogenic pulmonary edema, which of the following medications reduce venous return by means of water excretion?

One of the products of your annotation project was a dotplot comparing your annotated protein to the D. melanogaster ortholog (above). What does the yellow arrow indicate? What do the red and blue vertical stripes correspond to?

Dideoxy sequencing:  If nucleotides are fluorescently labeled as follows (dA, dG, dC,dT), what is the DNA sequence of the molecule being sequenced based on the gel shown below.  Note the samples are migrating in the gel   from top to bottom.?      Your answer should be all CAPS, no spaces.

Using the list of conditions, identify the PRIMARY condition for this image. [Example: The patient may have atelectasis, but if it’s caused by a pneumothorax, the pneumothorax would be the PRIMARY condition and atelectasis is a secondary result.] Conditions will not be used more than once. All conditions will not be used.   Cardiogenic Pulmonary Edema ARDS Lung Cancer Saddle Pulmonary Embolism Interstitial Lung Disease Subcutaneous Emphysema Plate-Like Atelectasis Left-Sided Atelectasis with Mediastinal Shift Right-Sided Atelectasis with Mediastinal Shift Left-Sided Pleural Effusion Right-Sided Pleural Effusion Left-Sided Pneumothorax with Mediastinal Shift Right-Sided Pneumothorax with Mediastinal Shift Flail Chest Image 3 93.jpg 

In your RNA analysis the goal of the project was to identify: