A researcher needs to isolate a rare CD34+ hematopoietic stem cell population (approximately 0.5% of total bone marrow cells) for downstream RNA sequencing analysis. Which technique would be MOST appropriate and why?

A researcher is selecting a primary antibody for Western blot detection of a 55 kDa protein. Which of the following antibody characteristics is MOST important to verify before purchase?

A researcher is selecting a primary antibody for Western blot detection of a 55 kDa protein. Which of the following antibody characteristics is MOST important to verify before purchase?

In the course of designing your expression strategy of your group’s protein, you decide to obtain an antibody to a specific epitope of the protein. What is the most efficient way to obtain a high-affinity, epitope-specific antibody?

You are looking at results from a flow cytometry experiment with two plots, A and B., representing the same results. At first glance A             B

For affinity purification of a protein from a bacterial lysate, which of the following collections would you expect to see the highest concentration of your desired (tagged) protein?

You recorded a flow cytometry experiment with a sample that had several cell populations in it. Plotting FSC vs. SSC which population (labeled in the graph as 1, 2 or 3) in this graph are the largest?  

What is the function of the blocking step in Western blotting?

When designing an expression plasmid for the gene assigned to your group, it is essential that:

Which region of an antibody is responsible for antigen binding?