You recreated experiments studying the lac operon in E. coli…
You recreated experiments studying the lac operon in E. coli (see schematic below). In your experiments, the activity of the enzyme β-galactosidase is visibly measured by addition of X- gal and IPTG into the growth media (lactose is present as well). X-gal is a lactose analog that turns blue when metabolized by β-galactosidase, but it does not induce the lac operon. IPTG is another analog of lactose that is an inducer of the lac operon but is not metabolized by enzyme β-galactosidase. In your experiment an E. coli without a functional “lac i” gene would be expected to ________.
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Answer the next four questions using the following sequence and Codon table ABOVE. Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a short hypothetical protein. Both strands are shown; the top strand reads 5’ to 3’ left to right, while the bottom strand reads 5’ to 3’ right to left. The nucleotides are numbered 1 to 100. Transcription begins with and includes the bold and underlined C/G (top strand/bottom strand) at position 9. Transcribe this gene into mRNA. What are the first 10 nucleotides of the resulting mRNA transcribed from this gene?
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