You seeded 10,000 cells in your 10cm in diameter plate on Monday. On Friday (day 4) you observe that your plate is 80% confluent, and you counted 160,000 cells. What is the population doubling time?

The growth of adherent cells in batch culture is limited by the amount of nutrients available rather than the amount of available surface area.

A student team is developing a tissue-engineered heart valve intended for use in a region with limited access to high-tech healthcare and strong cultural preferences for non-animal-derived medical products. Which of the following design decisions best reflects their ability to address cultural and social constraints?

Primary cell culture refers to cells. . .

You plan to evaluate biocompatibility of a material you implanted subcutaneously in a rodent model. After one week, you evaluate the tissue histologically. What staining method do you favor and why?

Flow cytometers analyze cells by microscopic visualization of cell populations.

Scanning electron microscopy (SEM) is useful to observe 3D characteristics of surface topography.

Macrophages will not form foreign body giant cells around a biocompatible material.

mRNA is synthetized from a DNA template in the

Primary protein structure refers to the sequence of amino acids that make up a protein.