You have identified a mutant in which some newly divided cel…
You have identified a mutant in which some newly divided cells die shortly after cell division, while others survive but exhibit an extra copy of one or more chromosomes. Interestingly, when the cells are cultured under conditions that slow down their progression through the cell cycle, the defect is no longer observed, suggesting that the mitotic spindle and chromosome segregation machinery are functioning normally. Which cell cycle checkpoint might be defective in this mutant? Provide a detailed explanation of your reasoning. Describe in detail the normal functioning of this checkpoint, including its role in maintaining genomic stability.
Read DetailsFor each of the following mutations, indicate whether the mu…
For each of the following mutations, indicate whether the mutation would likely promote (P) or inhibit (I) apoptosis in cells harboring the mutation(s). Your answer should be a four-letter string consisting of “P” and “I” only (e.g., PPPI). A mutation in the pro-apoptotic effector Bcl2 family proteins Bax and Bak that prevents their association with the outer mitochondrial membrane. A mutation in the BIR domain of the IAP protein DIAP1 that prevents binding to either caspases or anti-IAP proteins. A mutation in the anti-IAP protein Reaper that prevents its binding to IAP proteins. A mutation in the CARD domain of caspase-9 that prevents its binding to Apaf1.
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Complete the following sentences by filling in the blanks with the most appropriate term from the list provided. Note that not all terms will be used. apoptosome eat me phosphatidylserine cytochrome c initiator inhibitor extrapolar executioner condensation fragmentation Apaf1 In the intrinsic pathway of apoptosis, a protein called [response1] is released from the mitochondria into the cytosol and binds to the adaptor protein [response2], causing it to oligomerize into a wheel-like assembly called an [response3], which then recruits [response4] caspase-9 proteins. Next, [response5] caspases lead to the execution phase that results in DNA [response6] and corpse engulfment.
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