3. MICROSCOPY a. (2 pt) You want to image two fluorescent pr…
3. MICROSCOPY a. (2 pt) You want to image two fluorescent proteins within the same sample.mEGFP:Excitation max: 488nmEmission max: 507nmmCherry2:Excitation max: 589nmEmission max: 610nmAssume LEDs are used for excitation that precisely match the excitation maxima. Which type of filter would be best to use as an emission filter to detectmCherry2? a. Longpass filter with a 50% cut-off at 520nmb. Shortpass filter with a 50% cut-off at 600nmc. Bandpass filter with 50% cut-offs at 520nm and 660nmd. Bandpass filter with 50% cut-offs at 595nm and 625nm b. (3 pts) Briefly describe the components of a filter cube and their purposes.
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