Spleen cells are taken from a mouse immunized with the LCM v…
Spleen cells are taken from a mouse immunized with the LCM virus. 2 assays are used to determine antigen-specific functional activity of the spleen cells. Assay 1: the spleen cells (T cells) are incubated with macrophages that have been briefly exposed to LCM virus (phagocytized the virus), production of IL-2 by the T cells is considered a positive response. Assay 2: the spleen cells are incubated with LCM-infected target cells, lysis of the infected target cells is considered a positive response. The functional activity of which MHC molecules is detected in Assay 1?
Read DetailsTaking into account the selection process of developing T ce…
Taking into account the selection process of developing T cells in the thymus, why would somatic hypermutation in the genes of the TCR not be beneficial (remember that somatic hypermutation in the B cell antibody gene can take place after gene rearrangement and expression) to an individual and could lead to problems?
Read DetailsSpleen cells are taken from a mouse immunized with the LCM v…
Spleen cells are taken from a mouse immunized with the LCM virus. 2 assays are used to determine antigen-specific functional activity of the spleen cells. Assay 1: the spleen cells (T cells) are incubated with macrophages that have been briefly exposed to LCM virus (phagocytized the virus), production of IL-2 by the T cells is considered a positive response. Assay 2: the spleen cells are incubated with LCM-infected target cells, lysis of the infected target cells is considered a positive response. Activity of which cell population is detected in Assay 2?
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