The difference between detection and resolution is that you can resolve objects that you cannot detect.

For each of the following sentences, fill in the blanks with the best word or phrase selected from the provided lists. Not all words or phrases will be used; each word or phrase should be used only once. Affinity chromatographyForward Genetics Gel filtration chromatography GluaseHybridization Ion exchange chromatographyLigaseMultipotent cellsPluripotent cellsPrimary cell cultures Reverse Genetics Reverse Transcriptase Transformed cell linesUnipotent cellsWestern blotting

You are studying two novel proteins in migratory neural crest cells that you believe physically interact. Which of the following approaches is your best choice to visualize the proximity of these two proteins and evaluate the possibility of their interaction in live cells?

Caribou are adapted to live in the Arctic, which means their cells are functional in extreme cold. Based on your knowledge of cell membranes, which prediction would you make about the lipid bilayer of caribou cells?

Proteins run on SDS-PAGE gels are separated based on their size (mass) because SDS maintains intrinsic charge and beta-mercaptoethanol preserves three-dimensional structure.

You are in a lab that specializes in microscopy and you are studying breast cancer. Which microscopy technique would you use to perform the following experiments? For each experiment select the best possible technique from the drop-down menu. Use each technique only once. 

Symports and antiports always move one solute downhill (along its concentration gradient) and a second solute uphill (against its concentration gradient).

Using Fluorescence Recovery After Photobleaching (FRAP), you are studying the behavior of a membrane protein in red blood cells. Which FRAP graph would you expect to obtain if your protein is tethered/anchored to junctional complexes in the cortical cytoskeleton?  

There are three exams each worth 75 points.  Together these three exams count for _________ of my grade.

Membrane-associated proteins are embedded in the lipid bilayer through transmembrane alpha helices.