Explain your results for Test tube E3. How did the enzyme concentration influence the height of bubbles that were produced?

Part 3: Effect of Enzyme Concentration in Enzyme Activity  At a constant enzyme concentration and at lower concentrations of substrates, substrate concentration is the limiting factor. As the substrate concentration increases, the enzyme reaction rate increases. However, at very high substrate concentrations, the enzymes become saturated with substrate and a higher concentration of substrate does not increase the reaction rate. 

What is the optimum pH for your enzyme to function? 

Explain your results for Test tube E2. How did the enzyme concentration influence the height of bubbles that were produced?

Table 4: Effects of pH in Enzyme Activity

Hypothesis: Based on the information provided above come up with a hypothesis for your experiment.

Explain your results for Test tube 1. How did the temperature influence the enzyme activity?

Explain your results for Test tube 3. How did the temperature influence the enzyme activity?

Explain your results for Test tube P1. How did the pH influence the enzyme activity and its production of bubbles?

Part 2: Effect of temperature on Enzyme Activity  Background Information:  Each enzyme has an optimum temperature at which it works best. A higher temperature generally results in an increase in enzyme activity. As the temperature increases, molecular motion increases resulting in more molecular collisions. If, however, the temperature rises above a certain point, the heat will denature the enzyme, causing it to lose its three-dimensional functional shape by denaturing its hydrogen bonds. Cold temperature, on the other hand, slows down enzyme activity by decreasing molecular motion. In this experiment, you will explore this effect on the catalase extracted from a potato.