You extracted RNA using the organic solvent method (Trizol,…
You extracted RNA using the organic solvent method (Trizol, which uses phenol and chloroform to extract this molecule). You measured your extracted sample and the A260/A280 ratio is 1.7. You would describe this preparation as:
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After weighing the masses of Tris and EDTA you calculated in the previous question, you dumped the powders in a standard lab beaker, added ddH2O to the 100mL mark on the beaker and stirred until the powder was completely dissolved. Is this solution correctly made and ready for use as described (yes or no)? Justify your answer.
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