After completing the DNA extraction procedure on a patient specimen, you add the specimen extract to a well on an agarose gel (and in the well next to it, you add a molecular weight standard). After completion of the electrophoresis, you note that only the molecular weight standard is visible in the gel (and it has migrated as expected). Provide an explanation as to what might have occurred to create this result.  

What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.015 at 260 nm?

For successful recovery of DNA, extraction solutions must be within the pH range of 7-8. 

A plasmid is digested with the enzyme SmaI. On the agarose gel electrophoresis, you observe 2 bands: 250 and 300 bp. A. How many SmaI sites are present in this plasmid? (1 pt) B. What is the size of the plasmid? (1 pt) C. Now you cut the original (undigested by SmaI) plasmid with HindIII. The digestion yields 2 pieces, 50 and “X”. What is the “X” in base pairs? (1 pt)

RNA is isolated from a clinical sample. The absorbance at 260 nm is 0.525, and the absorbance at 280 nm is 0.257. Is this sample of sufficient quality for use in subsequent analyses? 

Which of the below storage conditions would be BEST for isolated RNA?

In organic isolation of RNA procedures, the main role of guanidinium isothiocyanate is to:

Which of the following gel matrixes is the best choice for separating two DNA fragments that are only one base pair apart?

Question 2:  Where did you struggle this semester? (This could be course related tasks, time management, or outside obstacles.) What solutions did you discover throughout the semester? What is your plan for future semesters to improve your own performance?

Juveniles can receive jury trials in some jurisdictions.