Given the following biochemical reactions, identify the organism. TSI = K/H2S ONPG = neg Citrate = neg Motility = pos Indole = pos Moeller Lysine = purple Moeller Ornithine = purple Moeller base = yellow MR / VP = pos / neg Mannitol = neg PAD = neg

An enteric organism is colorless on MAC and yellow/black on TSI. Completely interpret these reactions:

An outbreak of severe diarrhea occurred 12-72 hours after a holiday party where the gazpacho was linked to infection in almost everyone who attended. In stool cultures from the patients the pathogen grew medium to large clear colonies on MAC and XLD plates. Based on the additional biochemical reactions below, what is the most likely identification of the organism causing the outbreak? Citrate = negative Indole = negative Mannitol = positive Motility = negative Moeller Lysine = yellow Moeller Ornithine = yellow Moeller Base = yellow MR = positive ONPG = negative Oxidase = negative PAD = negative TSI = K/A Urea = negative

An otherwise healthy 69-year-old male presents to his physician with fever and shortness of breath. Chest x-ray reveals a right lower lobe pneumonia. Gram stain of an expectorated sputum specimen show many neutrophils, few squamous epithelial cells, rare Gram-positive cocci in chains, and many plump Gram-negative diplococci. Sputum culture reveals few small, round alpha hemolytic colonies and many medium, tan gamma hemolytic colonies on BAP. The growth on CHOC plate is similar to BAP. MAC has no growth. When a tan colony is touched with an inoculating loop it slides on the plate like a hockey puck. Oxidase and butyrate test on the tan colonies are both positive. What organisms is causing this man’s pneumonia?

Given the following biochemical reactions, identify the organism: TSI = yellow / yellow Indole = neg Motility = pos Citrate = pos DNAse = neg MR / VP = neg / pos Moeller ornithine = purple Moeller lysine = yellow Moeller base = yellow PAD = neg

Modified Thayer-Martin agar is prepared with hemoglobin base, supplemented with enrichments and:

A foot ulcer from a diabetic patient grew oxidase negative bacilli that were lactose negative on MacConkey.  The following biochemical tests were performed:   Phenylalanine deaminase: Negative Citrate: Positive SIM: 0, 0, +  MR/VP: 0/+ Lysine decarboxylase: Negative Ornithine decarboxylase: Negative DNase: Negative ONPG: Positive Arabinose: Positive The organism should be identified as:

Match the test to the to the reagent

Match the organism with the disease it most commonly causes.

An organism is alkaline /acid on triple sugar iron agar.  This indicates that it: 1. Cannot ferment glucose 2. Ferments glucose  3. Ferments lactose or sucrose 4. Ferments sucrose