Consider an association between a Customers class and a Sale class in a Unified Modeling Language (UML) Class diagram. The multiplicities next to the Customers class are 1..1 and the multiplicities next to the Sale class are 0..*. Which of the following is the best way to implement that association in your database?

miRNA expression is required throughout development.  Explain how miRNA expression is utilized during the maternal to zygotic transition why miRNA expression is required for proper development Make sure to address both bullet points

Describe 1 similarities and 1 differences between chromatin immunoprecipitation profiling vs RNA profiling

VEGF (vascular endothelial Growth Factor) is a signal that is involved in differentiating cells into blood vessels where as NGF (Neural Growth Factor) differentiates neurons, and EGF (Epithelial Growth Factor) differentiates skin cells. You have a zebrafish that has EGF promotor in front of GFP in the DNA.  Design an experiment in which you determine if the first 10 green cells you see are specified.

Describe 2 similarities and 1 difference between how ions are used in a sperm vs an egg during fertilization.

VEGF (vascular endothelial Growth Factor) is a signal that is involved in differentiating cells into blood vessels where as NGF (Neural Growth Factor) differentiates neurons, and EGF (Epithelial Growth Factor) differentiates skin cells. You have a zebrafish that has NGF promotor in front of GFP in the DNA.  Design an experiment in which you determine if the first 10 green cells you see are specified.

The frog life cycle below is labeled with a question marker (???). Use the drop downs from the question to answer in relation to the cycle that is marked by the question symbol. “In the [option1] stage of the cycle, at the beginning [option2]. Once this event occurs the cell begins to be referred to as a [option3].”   

You recently found out that the exact spot in which the head forms in a developing fish at gastrulation. You scooped out that area of the embryo and one on the opposite side of the embryo and did an RNA sequencing run on both tissues. After analyzing your data you have seen that there is a large increase in b-catenin where the head is formed compared to the opposite side.  Design an experiment in which you determine if b-catenin is REQUIRED for head formation.

Convert `X`F to Kelvin

 A certain ocean wave has a frequency of 0.05 Hz and a wavelength of 10 m. What is the wave’s speed? (Answer in m/s)