In class we discussed the paper, “Optogenetics enables funct…
In class we discussed the paper, “Optogenetics enables functional analysis of human embryonic stem cell-derived grafts in a Parkinson’s disease model”. The figures below come from that paper. (FYI: Halorhodopsin-EYFP is the same thing as eNpHR3.0-EYFP) Match each panel (A, B, and C) with the mouse genotype that could be responsible for the data.
Read DetailsThe figure below is a modified figure from the paper, “Optog…
The figure below is a modified figure from the paper, “Optogenetics enables functional analysis of human embryonic stem cell-derived grafts in a Parkinson’s disease model”. Which two bars in the figure denote the behavior of “diseased” mice before dopamine cell transplantation?
Read DetailsPretend you are scientist studying neurons that are growing…
Pretend you are scientist studying neurons that are growing in a culture dish. You use Fura-2 to measure the intracellular calcium concentration of these neurons. You measure the fluorescence intensity at Time 1 and obtain a ratio indicative of the red trace. Next, you puff a chemical onto the neurons. Immediately after the puff, at Time 2, you again measure the fluorescence intensity. At Time 2 you obtain a ratio indicative of the blue trace. How did the chemical puff affect the neurons?
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