Below is a sequence of 100 base pairs from the ADE2 gene of…
Below is a sequence of 100 base pairs from the ADE2 gene of yeast, and six 8 base oligonucleotide primers. You wish to amplify portions of the ADE2 gene using PCR. Which pairs of primers (oligos) will give you a PCR product if you use the given ADE2 genomic DNA as a template? how long will the product be? Write how long will the PCR product be from each primer pair listed below (the answer is number only!). If the listed primer pair results in no PCR product, write 0. Primer pair 1+2 will give PCR product length: [1] Primer pair 1+3 will give PCR product length: [2] Primer pair 1+4 will give PCR product length: [3] Primer pair 1+5 will give PCR product length: [4] Primer pair 1+6 will give PCR product length: [5] Primer pair 2+3 will give PCR product length: [6] Primer pair 2+4 will give PCR product length: [7] Primer pair 2+6 will give PCR product length: [8] Primer pair 4+5 will give PCR product length: [9] Primer pair 3+4 will give PCR product length: [10] Primer pair 3+5 will give PCR product length: [11] Primer pair 3+6 will give PCR product length: [12]
Read Details