Many young animals are _____, a sexually immature form that is morphologically distinct from the adult stage. These young animals undergo _____ to become _____, which resemble adults but are not yet sexually mature.

RPE65 is a major protein of unknown function found associated with the human eye that is associated with a number of different eye diseases. One hypothesis is that RPE65 is an enzyme involved in the chemical synthesis of the pigment that makes the human eye light sensitive. In a search as to which pathway intermediate RPE65 acts on, the following dissociation constants were measured (Table 1). They were compared to BSA, which stands for bovine serum albumin and is found in large concentrations in cow milk. Table 1:  Binding Constants of Various Retinoids with RPE65 and BSA in pM (10^-12 M). The numbers in brackets (  ) indicate the errors of the binding constant determination. Ligands RPE65 (pM) BSA (pM) A: all-trans-retinyl palmitate 20 (6) 2143 (790) B: 11-cis-retinyl palmitate 14224 (3006) 2310 (611) C: all-trans-retinol 1078 (144) 2316 (1216) D: 11-cis-retinol 3811 (100) 2143 (800)   Part A. 4 points What is the purpose of comparing the dissociation constants of 4 different ligands with two different proteins, RPE65 and BSA?  Part B. 5 points What is the association constant for 11-cis-retinol binding to RPE65? Part C. 5 points What is the difference in standard binding free energy between ligand A binding to RPE65 as compared to BSA. Part D. 4 points Which is the best (highest affinity) ligand for RPE65? Justify your choice. Part E. 4 points Would you say that RPE65 binding to any of the ligands is specific? Justify your answer. Part F. 10 points If you add a 10 nanomolar concentration of ligand A to a solution containing RPE65, what percent of ligand A is bound to RPE65? Part G. 10 points If you repeat the same experiment as in part F with BSA, i.e. if you add a 10 nanomolar concentration of ligand A to a solution containing BSA, what percent of ligand A is bound to BSA? Solve this problem on paper, document your approach and provide intermediate results.  Report the final answers with 3 significant figures.  Scan the pages(s) and upload them for grading. Answer all parts A to G.

Which one of the following is true of seed plants, but NOT true of seedless plants?

A preterm newborn who weighs 2400 grams is being mechanically ventilated on PCV with minimal chest rise. The following ABG was obtained on the following ventilator parameters. What should be done?PCV                                      pH = 7.24f = 30                                     PaCO2 = 60 mmHgpressure = 35 cm H2O            PaO2 = 62 mmHgFIO2 = 40%                           HCO3 = 26 mEq/LPEEP = 5 cm H2OEx. Vt = 14.0 ml

Which assumptions for an ideal gas are relaxed in the van der Waals model of a real gas.

Which is a key difference between alternation of generations in plants and sexual reproduction in nonplant organisms?

According to the punctuated equilibrium model of evolution, _____.

Lake Victoria, in Africa, is home to a group of related fishes known as cichlids. Many of these fishes are similar in appearance but have different feeding habits. What is the best method for scientists to determine conclusively whether the fish are members of a population that has a lot of variation or members of entirely different species?

Each pollen grain of a gymnosperm contains a _____.

A chemical reaction has an activation energy of 44.422 kJ/mol and its initial (forward) reaction rate has been determined at 25 °C. How will the reaction rate change, when the temperature is decreased to 12.5 °C?