In Anfinsen’s ribonuclease refolding experiment, ribonucleas…
In Anfinsen’s ribonuclease refolding experiment, ribonuclease A—an enzyme containing 8 cysteine residues that form 4 disulfide bonds—was first fully denatured using urea and 2-mercaptoethanol and then allowed to refold by removing these agents. Which of the following conclusions is best supported by the results of this experiment?
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