Kirby-Bauer susceptibility testing requires: 1. organisms in suspension with a turbidity equal to a 5.0 McFarland standard. 2. organisms with a rapid rate of growth. 3. standardized depth of Mueller-Hinton agar. 4. incubation in increased CO2 incubator.
Read DetailsBoth sets of blood cultures from a 44 year-old male patient (post appendectomy) grew Gram negative bacilli from both the aerobic and anaerobic bottles after 18 hours incubation. Biochemical reactions demonstrate the following: MAC = pink donuts BAP = luxuriant growth TSI = acid / H2S Indole = negative Motility = positive Citrate = positive MR/VP = +/0 Ornithine = negative Lysine decarboxylase = negative PAD = negative The most likely genus and species of this organism is:
Read DetailsA Gram negative bacillus was isolated from the CSF of an infant in the neonatal ICU. The infant was born 18 days ago at an estimated gestational age of 27 weeks. Biochemical reactions demonstrate the following: TSI = acid/acid no H2S Lysine decarboxylase = purple Indole = positive Arginine dihydrolase = yellow Citrate = peacock blue Ornithine decarboxylase = yellow MR/VP = 0/+ Decarboxylase base = yellow Urea = Magenta pink PAD = Negative Motility = Negative The most likely genus and species of this organism is:
Read DetailsThese images are from the culture of an expectorated sputum specimen from a 67 year old patient suffering from symptoms of pneumonia (BAP on the left, CHOC on the right, MAC showed no growth). What are the next steps in working up this culture?
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