A PCR assay on DNA isolated from a nasopharyngeal swab was performed with the following results: amplification using primers to IS481 was positive and using primers to IS1001 was negative. All controls were acceptable. Which of the following organisms was present in this sample?

Whole blood was sent to the laboratory for viral load determination of HIV (an RNA virus). It was received on a Saturday morning, and the technologist working in the receiving section of the laboratory put the tube in the refrigerator at 2o-8oC. The molecular technologist came on Monday morning and collected the specimens that had been sent to the laboratory over the weekend. How should this sample be handled on Monday morning?

The hemochromatosis mutation C282Y is which of the following mutations?

A blood specimen is received in the laboratory for a PCR assay to detect cytomegalovirus (a DNA virus) on a Wednesday night. The PCR assay will not be performed until the next Monday morning. How should this specimen be stored to maintain the integrity of the nucleic acid prior to analysis?

A woman calls and asks that the results of her husband’s HIV viral load assay be faxed to her. She gives the technologist her husband’s name and medical record number along with the date the specimen was collected and her fax number. She states that she is calling for her husband and has his approval. Which of the following is the correct course of action for the technologist?

A patient with abnormally high iron levels is tested to determine if hemachromatosis, specifically the C282Y mutation in the HFE gene, is the cause. The patient’s DNA is isolated from whole blood and amplified. Amplicons are then cut with Rsa1, and the resulting fragments are separated by gel electrophoresis. The lane containing the patient’s sample has three bands at 240 bp, 140 bp, and 110 bp. Controls were acceptable. Which of the following is the correct interpretation of these results?

A quantitative method was performed 100 times, and results from 99 of the 100 runs were in agreement with each other. This method has 99%:

From the case study in the previous question (#97), If the modified method had been validated, what should have been done with the PT samples? 

The purpose of endonucleases is to:

Which of the following translocations is associated with T-cell lymphoma?