Are the cells in the sample likely to be alive or dead after using the methods described below? 1. Fluorescence microscopy is used to visualize a GFP fusion protein that expressed in cells. [ans1] 2. Immunofluorescence is used to stain for an intracellular protein. [ans2] 3. Flow cytometry is used to visualize a transmembrane protein labeled on the exterior of cells with a fluorescent antibody. [ans3]
Read DetailsNeuropeptides are small proteins/peptides that must be released by neurons so they can communicate with other (post-synaptic) cells. A. What type(s) of amino acid signal sequence(s) would you expect to find on a neuropeptide? B. After these neuropeptides have been released, they are frequently collected and reused by the same neurons. What must happen to the neuropeptides in order for them to be reused? (Describe what happens to them after they have been secreted.)
Read DetailsIn order to estimate the average electric usage per month, a sample of 196 houses was selected and their electric usage was determined. a. Assume a population standard deviation of 350 kilowatt-hours. Determine the standard error of the mean. [popstddev] b. With a .99 probability, determine the margin of error. [answerb] c. If the sample mean is 2100 kWh, what is the 98% confidence interval estimate of the population mean? [answer1] to [answer2] kWh
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