Which of the following controls are critical for ensuring that amplification is occurring in a patient sample and that lack of PCR product is not due to the presence of polymerase inhibitors?

Amplification of the polA is highly indicative of which of the following?

Which virus does not share sequence homology with the others?

Which will change with environment and age?

Which of the following involves using flow cytometry to  measure mRNA overexpression?

What characteristic of parasites complicates molecular testing?

The mutation del(15)(q11q13) on the paternal chromosome results in a disease that is different from when the same mutation occurs on the maternal chromosome. This difference results from which of the following?

A patient with abnormally high iron levels is tested to determine if hemochromatosis, specifically the C282Y mutation in the HFE gene, is the cause. The patient’s DNA is isolated from whole blood and amplified. Amplicons are then cut with RsaI, and the resulting fragments are separated by gel electrophoresis. There are no RsaI sites in the normal 250 bp PCR product, while the C282Y mutation generates one RsaI site. The lane containing the patient’s sample has three bands at 250 bp, 140 bp, and 110 bp. Controls were acceptable. Which of the following is the correct interpretation of these results?

A (F508del) mutation in the CFTRgene is responsible for causing which of the following diseases?

PCR with RFLP analysis and reverse hybridization is the principle of which of the following commercial assays?